葡萄糖测定试剂盒(己糖激酶法)

葡萄糖6磷酸脱氢酶

 
PRODUCT DESCRIPTION
Appearance White amorphous powder, lyophilized
Source Microorganism
Molecular Weight 56 kDa (SDS-PAGE)
Theoretical PI pH 5.1
EC Number EC 1.1.1.49
CAS Number 9001-40-5
 
PROPERTIES
Specific Activity More than 100 U/mg solid at 37 oC
Michaelis Constant D–Glucose–6–phosphate 2.6×10-3 M
  NAD+ 3.9×10-4M
Optimum pH 8.0 (Fig.1)
pH Stability 6.0~11.0 (25 oC, 20hr) (Fig.2)
Thermal Stability <40oC (pH 8.2, 10 min) (Fig.3)
Storage Stability At least one year at -20 oC (Fig.4)
  At least one year at 4 oC in HEPES
         
 
 
APPLICATIONS
The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase (MB-HK).

 
Table 1.
Effect of Various Chemicals on G6PDH
 
Chemicals Concn. Activity   Chemicals Concn. Activity
None -- 100%   EDTA-2Na 5mM 98%
NaCl 2mM 99%   NaN3 2mM 99%
KCl 2mM 100%   NaF 2mM 99%
CaCl2 2mM 99%   Proclin 300 0.1% 98%
MgCl2 6H2O 2mM 97%   Brij-35 0.1% 59%
BaCl2 2mM 92%   Tween-20 0.1% 65%
CuCl2 2H2O 2mM 102%   cholate acid 0.1% 3%
MnCl2 2mM 70%   Trition X-100 0.1% 91%
 
 

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